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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Invest Dermatol. 2014 Nov 28;135(4):1043–1052. doi: 10.1038/jid.2014.505

Figure 1. ACTN1 and ACTN4 expression and localization in keratinocytes.

Figure 1

(a) Extracts of iHEKs, three clones of iHEKs infected with virus encoding ACTN1 shRNA (ACTN1shRNA-A, -B and -C) and iHEKs infected with virus encoding scrambled shRNA (scrambled shRNA) were processed for immunoblotting using antibodies against ACTN1, ACTN4 and lamin A/C as indicated. Lamin A/C reactivity was used as a loading control. Blots were scanned and quantified by densitometry, values were normalized to lamin A/C levels and are displayed relative to iHEKs levels. The blot is representative of at least three independent trials. (b) iHEKs, iHEKs expressing scrambled shRNA and iHEKs expressing ACTN1 shRNA were prepared for immunofluorescence staining either with ACTN1 antibodies (upper panels) or ACTN4 antibodies (lower panels). Note that the actinin-1 antibody stains the nucleus. This staining is not lost following actinin-1 knockdown indicating that it is non-specific. Arrowheads indicate filopodia and arrows lamellipodial extensions. Bars in images, 10 µm.