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. Author manuscript; available in PMC: 2016 Mar 16.
Published in final edited form as: Curr Biol. 2015 Feb 12;25(6):764–771. doi: 10.1016/j.cub.2015.01.034

Figure 4. DMRT1 can silence FOXL2 without SOX8 and SOX9.

Figure 4

(A-C) CAG-Stop-Dmrt1-Gfp can silence FOXL2 in Sox9 conditional mutant ovaries. IF showing that SOX9 is expressed in Sertoli cells of wild-type testes and in Sertoli-like cells of control conditional Sox9/+ DMRT1-expressing ovaries. FOXL2 is almost completely silenced in DMRT1-expressing ovaries conditionally deleted for one (B) or both (C) copies of Sox9 with Sf1-Cre. (D-G) CAG-Dmrt1-Gfp can activate SOX8 in SOX9 mutant granulosa cells. IF showing that SOX8 is expressed in Sertoli cells in wild-type adult testes (D) and is not detectable in wild type adult ovaries (E). CAG-Dmrt1-Gfp can activate SOX8 in ovaries conditionally deleted for one copy (F) or both copies (G) of Sox9 in somatic cells using Sf1-Cre. (H-J) CAG-Dmrt1-Gfp can activate the mature Sertoli cell marker GATA1 in Sox9 mutant granulosa cells. IF showing that GATA1 is not expressed in wild type adult ovaries (H) but is expressed in ovaries conditionally deleted for one (I) or two (J) copies of Sox9 in somatic cells using Sf1-Cre. (K-M) DMRT1 silences FOXL2 in granulosa cells lacking both Sox8 and Sox9. IF showing that Sox8;Sox9 double mutant ovaries have normal FOXL2 expression, normal morphology and lack DMRT1 (K). Activation of CAG-Dmrt1-Gfp in ovaries heterozygous for Sox8 and Sox9 (L) or homozygous mutant for both genes in somatic cells (M) can induce DMRT1 expression and silence FOXL2. Scale bars: 40 μm (A-D,F-J); 20 μm (E,K-M)