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. Author manuscript; available in PMC: 2015 Oct 1.
Published in final edited form as: J Invest Dermatol. 2014 Dec 18;135(4):1089–1097. doi: 10.1038/jid.2014.528

Figure 3. BAP1 depletion causes cell cycle arrest and apoptosis.

Figure 3

Analysis of cell cycle progression and apoptosis were performed at 6 or 7th day following shRNA-mediated BAP1 silencing. Cell cycle analysis in (a) 10% and (b) 2.5% serum medium using control cells (gray shading) and shBAP1 knockdown cells (hatched shading). Error bars represent SEM from triplicate samples and 3 experimental replicates are shown. A375 and SKmel-119 are cutaneous melanomas and C918 is an ocular melanoma used for comparison; *p<0.05, **p<0.01 by student T test. (c) FITC-Annexin staining of cultured control and BAP1-depleted cells in both 10% and 2.5% serum. DNA fragmentation may underestimate the level of apoptosis, especially if the total DNA content is elevated from G2/M arrest. Error bars represent SEM from triplicate samples both replicates shown; **p<0.01 by student T test.