Fig 1. Compound 2 enhances differentiation.

A. Structure of Compound 2. B. Immunofluorescence of a cyst-like structure using an anti-Toxoplasma antibody (green) to detect all parasites and Dolichos biflorus lectin (DL; red) to detect the cyst wall after 72 hr under CO₂ starvation conditions. The corresponding differential interference contrast (DIC) and merged images are also shown. Scale bar = 10μm. C. Percentage of DL-positive vacuoles (DL+) in samples treated with either Compound 2 (3μM) or an equivalent volume of DMSO for 72 hr under CO₂ starvation conditions (mean ± SD; n = 4 for the wild-type strain and n = 6 for Δuprt strains). The data were compared using paired Student’s t-test (**p < 0.01).