Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Mar 19;10(3):e0120331. doi: 10.1371/journal.pone.0120331

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 Odell et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Fig 2 — A. Schematic of the Y3H system showing the ternary complex between the CID and the two fusion proteins containing the activation and DNA-binding domains of the transcription factor (AD and DBD, respectively). The CID consists of: methotrexate (green cross), which binds to the DHFR-DBD fusion; a flexible linker unit (red); and the small molecule of interest (blue star), which binds to the target protein-AD fusion. B. Synthetic route to MTX-Cmpd2.1 (i) Compound 2-PEG linker fragment 3; (ii) ^tButyl Methotrexate 5 and (iii) Peptide coupling and ^tbutyl deprotection to MTX-Cmpd2.1. For reagents and conditions, see S1 Methods.