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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Mol Cancer Ther. 2013 Oct 29;13(1):221–229. doi: 10.1158/1535-7163.MCT-13-0561

Figure 1. Sorafenib suppresses UV-induced apoptosis.

Figure 1

HaCaT and NHEK cells were either unirradiated or irradiated with 500 J/m2 of UVB in the absence (“o”, 1:2000 DMSO) or presence (“+”) of 1 μM sorafenib and isolated for FACS analysis and protein extracts 24 hours later. A, HaCaT, B, NHEK cells show 45% and 80% suppression, respectively, of apoptosis in the presence of sorafenib as measured by FACS for Annexin V+, TMRE-low cells (n=6 for each condition, “***”, p<0.001). Western blots of HaCaT (C) and NHEK (D) cells probed for the MAP kinases JNK and ERK demonstrated strong phospho-JNK induction following irradiation and significant suppression by sorafenib. Phospho-ERK was slightly suppressed following irradiation, and at all time points, paradoxical hyperactivation in the presence of sorafenib was observed, particularly in HaCaT cells. Importantly, phospho-MKK4 and phospho-MKK7, upstream kinases that activate JNK, were both strongly induced by UV radiation and strongly suppressed by sorafenib.