Skip to main content
. 2015 Mar 5;6:6456. doi: 10.1038/ncomms7456

Figure 3. KillerRed photoactivation and degradable transgene expression.

Figure 3

(a) Fluorescence intensity of ROS generation by KillerRed-positive cells incubated at different light exposure time periods (593 nm, 100 mW cm−2), analysed by flow cytometry. The cells were transfected with DNA-complexes containing only plasmid DNA of KillerRed at pH 6.8. Results show the mean of the measurements conducted in triplicate±s.d. (b) Results of the viability and apoptosis of KillerRed-positive cells incubated at light exposure time points determined by the MTS assay and TUNEL assay. Results show the mean of the measurements conducted in triplicate±s.d. The untreated cells were used as a control. (c) Kinetics of gene expression in cells transfected with the DNA-complex (weight ratio of 0.5/1/1) at pH 6.8. Results show mean of measurements conducted in triplicate±s.d.