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FAK phosphorylates vitronectin, PTPN5, and PDCD6. FAK-IVK reactions with VTN (a), GST-PTPN5 (b), GST-PDCD6 (c and e), using GST or GST-paxillin as negative or positive controls, respectively. Left panels (a-c): stained proteins; right panels: autoradiographs of FAK-IVK. d. IVK with purified Src or Pyk2 using GST, GST-paxillin, or GST-PTPN5 as substrates. e. IVK reactions with purified FAK, Src or Pyk2 using GST, GST-paxillin or GST-PDCD6 as substrates.
