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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Xenotransplantation. 2014 Jul 2;21(4):376–384. doi: 10.1111/xen.12106

Figure 2.

Figure 2

Expression of Gal and Neu5Gc on erythrocytes by flow cytometry. Erythrocytes from Dom, GGTA1 KO and GGTA1/CMAH KO pigs, baboons, chimpanzees, and humans were labeled with fluorescently conjugated Sambucus nigra (SNA) lectin (red line) to detect Neu5Ac linked α 1,6 galactose expression (top panel) were used as a positive control for the presence of erythrocytes. Fluorescently conjugated IB4-lectin was used to assess Gal expression (middle panel)(red line), and anti-Neu5Gc antibody was used to examine the expression of Neu5Gc epitopes (bottom panel)(red line). Unstained erythrocytes are shown as negative controls (black line) for SNA and IB4-lectins, and an isotype control for Neu5Gc. Histograms are representative of 3 individual experiments.