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. Author manuscript; available in PMC: 2015 Jul 2.
Published in final edited form as: Xenotransplantation. 2014 Jul 2;21(4):376–384. doi: 10.1111/xen.12106

Figure 5.

Figure 5

Flow cytometry analysis of human or baboon IgG/IgM binding to erythrocytes. A 4-fold serial dilution of human or baboon sera was used to label erythrocytes from Dom, GGTA1 KO and GGTA1/CMAH KO pigs, as well as baboons, chimpanzees, and humans. Bound human and baboon antibody was detected with fluorescently conjugated anti-human IgG or IgM antibodies. Erythrocytes from each group were treated with secondary antibody only as a control. Histograms shown are representative of 3 experiments (Panel A). IgG/IgM binding was assessed after normalization with the MFI of erythrocytes incubated with only secondary antibody. The results from individual experiments using 6.3% final sera are expressed as mean ± SEM, n=3 and *p<0.05 (Panel B).