Figure 6.
a) Protein expression of albumin was confirmed by western blot analysis after treatment of rat derived omentum tissue mesenchymal stem cells with 6 µg/ml liver extract. Undifferentiated rat derived omentum tissue mesenchymal stem cells as negative control (NC), 16 day differentiated cells and 21 day differentiated cells. b) Periodic acid-Schiff staining for glycogen production in the differentiation process of rat derived omentum tissue mesenchymal stem cells in presence of 6 µg/ml liver extract. Unstaining differentiated cells after 16 days (a), differentiated cells after 16 days (b), and 21 days (c). c) Urea secretion by rat derived omentum tissue mesenchymal stem cells was detected at various times throughout differentiation. Undifferentiated rat derived omentum tissue mesenchymal stem cells as negative control (NC), 16, 18 and 21 day differentiated cells, cell line HepG2 as positive control (PC)