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. 2015 Apr;87(4):747–765. doi: 10.1124/mol.114.096495

Fig. 4.

Fig. 4.

Effects of CRIP1a overexpression on concentration-effect curves of ligand-modulated [35S]GTPγS binding in CB1-HEK cell membranes. Membranes from CB1-HEK cells with (open symbols) and without (closed symbols) stable cotransfection of CRIP1a were incubated (as described in Materials and Methods) with 100 mM NaCl, 10 μM GDP, 0.1 nM [35S]GTPγS, and varying concentrations of the indicated ligands: (A) noladin ether (2-AGE) and CP55,940 (CP); (B) WIN55,212-2 (WIN) or methanandamide (MAE); or (C) ∆9-tetrahydrocannabinol or rimonabant (RIM). Data are mean percentage stimulation ± S.E.M. (n = 3–6). No CRIP1a, no transfection of CRIP1a; CRIP1a, stable transfection of CRIP1a.