Fig. 4.

Effects of CRIP_1a overexpression on concentration-effect curves of ligand-modulated [³⁵S]GTPγS binding in CB₁-HEK cell membranes. Membranes from CB₁-HEK cells with (open symbols) and without (closed symbols) stable cotransfection of CRIP_1a were incubated (as described in Materials and Methods) with 100 mM NaCl, 10 μM GDP, 0.1 nM [³⁵S]GTPγS, and varying concentrations of the indicated ligands: (A) noladin ether (2-AGE) and CP55,940 (CP); (B) WIN55,212-2 (WIN) or methanandamide (MAE); or (C) ∆⁹-tetrahydrocannabinol or rimonabant (RIM). Data are mean percentage stimulation ± S.E.M. (n = 3–6). No CRIP_1a, no transfection of CRIP_1a; CRIP_1a, stable transfection of CRIP_1a.