Fig. 5.
CRIP1a overexpression in CB1-HEK cells suppressed basal [35S]GTPγS binding in the absence of sodium in a PTX-sensitive manner. Membranes from CB1-HEK cells with and without stable cotransfection of CRIP1a were pretreated for 24 hours with and without 50 ng/ml PTX and were then incubated with 10 μM GDP and 0.1 nM [35S]GTPγS in the presence and absence of 100 mM NaCl (as described in Materials and Methods). Data are mean fmol/mg bound ± S.E.M. (n = 4). *P < 0.05 different from cells without CRIP1a cotransfection under the corresponding conditions, as determined by two-way ANOVA with Bonferroni posthoc test. No CRIP1a, no transfection of CRIP1a; CRIP1a, stable transfection of CRIP1a.