TABLE 2.
CB1-HEK | CB1-HEK-CRIP1a | ||||
---|---|---|---|---|---|
Ligand |
Emax |
EC50 |
Emax |
EC50
|
P value |
% Stim | nM | % Stim | nM | ||
2-AGE | 137.7 ± 9.7a | 59.9 ± 3.8 | 108.2 ± 3.7a,* | 104 ± 16 | <0.0001 |
HU210 | 114.6 ± 5.9a,b | 0.04 ± 0.01 | 96.0 ± 6.1b,§ | 0.06 ± 0.01 | <0.0001 |
WIN55,212-2 | 112.7 ± 8.8a,b,c | 110 ± 41 | 79.5 ± 1.7c,* | 90.6 ± 6.2 | <0.0001 |
CP55,940 | 100.0 ± 8.7b,c | 48.8 ± 2.2 | 72.0 ± 2.7c,* | 51.4 ± 6.3 | <0.0001 |
MAEA | 73.8 ± 6.1c | 202 ± 37 | 73.0 ± 6.0c | 255 ± 40 | 0.4928 |
Levonantradol | 73.7 ± 11.3c | 34.2 ± 8.6 | 72.8 ± 0.5c | 19.0 ± 5.2 | 0.2360 |
THC | 19.6 ± 2.7d | 11.5 ± 2.4 | 20.4 ± 3.1d | 8.1 ± 1.2 | 0.1740 |
Rimonabant | −12.9 ± 1.5 | 1.0 ± 0.4 | −6.5 ± 1.5* | 0.5 ± 0.1 | 0.0002 |
P < 0.05 different from CB1-HEK cells. §P = 0.05 different from CB1-HEK cells. a–dSignificance of Emax and EC50 values between cell lines were determined by Student's t test. Significant differences between ligand Emax values within each cell line are denoted as follows: ligands without any similar letter designations are P < 0.05 different from each other as determined by one-way ANOVA with posthoc Newman-Keuls test.