Fig. 7.

SKA-31 normalized 4-AP (3 μM) triggered dysregulation of Ca²⁺ dynamics in HN cultures at 9 DIV. (A) Representative traces showing how SKA-31 influenced 4-AP–triggered Ca²⁺ dynamics. (B) SKA-31 attenuated 4-AP–induced acute rise in cytoplasmic Ca²⁺. (C) SKA-31 attenuated 4-AP–triggered increases in SCO frequency. (D) SKA-31 attenuated 4-AP–induced decreases in SCO amplitude. Each data point represents at least three replicates, and the experiments were repeated twice in independent cultures with similar results. AUC, area under the curve. **P < 0.01, 4-AP versus Veh (vehicle); ^##P < 0.01, SKA 31+4-AP versus 4-AP.