Fig. 2.
Western blots of 2C-Fab-GFP. (A) Lane 1: molecular mass markers (kilodaltons); lane 2: 2C-Fab-GFP purified from the culture media of a stable HEK293 cell line coexpressing the heavy- and light-chain plasmids. The light chain, heavy chain, and GFP are approximately 30 kDa each. (B) Lane 1: molecular mass markers (kilodaltons); lane 2: total protein extract from choroid plexus tissue labeled with 2C-Fab-GFP. Bands within the predicted ranges for immature to mature 5-HT2C receptors (45–60 kDa), homodimers (90–120 kDa), and homodimers in complex with G protein (170–200 kDa) are present.