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Cells were pretreated with 50 nM 5-HT, 10 μM Cab, or 25 nM Erg for 60 minutes. Following drug washout, IP production was measured in the absence (−) or presence of 50 nM 5-HT or 1 μM mianserin (Mian) for 60 minutes. Data are expressed as the percentage of maximal IP production achievable with each drug, with basal IP levels subtracted out. Data represent the mean ± S.E.M. from four to six experiments. *P < 0.01 versus no drug (−).
