Table 2. Toxins and antitoxins of S. Typhimurium TA modules detected by mass spectrometry in bacteria isolated from fibroblasts and in bacteria grown in LB medium.

			Extracellular (†)			Intracellular (‡)
Accesion Number	Protein (*)	Mass (Da)	Unique peptides	PSMs	Coverage (%)	Unique peptides	PSMs	Coverage (%)
SL1479	RelEST	11,070	3	8	29.79	1	4	9.57
SL1480	RelBST	9,086	1	2	23.17	-	-	-
SL2379	A1ST	13,536	4	19	33.33	-	-	-
SL2380	T1ST	14,966	4	6	34.62	-	-	-
SL2658	PasIST	10,769	3	6	31.25	-	-	-
SL2659	PasTST	17,725	2	3	13.29	-	-	-
SL2884	A2ST	10,673	2	5	28.13	2	2	31.25
SL2885	T2ST	19,059	2	4	13.14	2	4	20.00
SL2936	ParDST	10,369	-	-	-	1	1	7.69
SL3011	VapCST	14,930	-	-	-	1	1	21.21
SL3012	VapBST	14,276	2	3	13.60	1	2	6.40
SL3437	T3ST	22,767	11	27	68.00	8	27	47.00
SL3484	DinJST	9,442	2	4	24.42	-	-	-
SL3524	DocST	13,580	1	2	7.38	1	2	10.66
SL3617	T4ST	17,693	2	5	22.36	1	3	8.07
SL3744	RelB3ST	11,754	4	6	36.45	-	-	-
SL3976	SehBST	15,915	1	2	7.75	-	-	-
SL3979	SehCST	12,326	1	1	8.33	-	-	-
SL4253	A5ST	10,972	1	2	9.28	2	4	23.71
SL4254	T5ST	17,636	1	1	7.98	1	6	7.98
SL4460	ShpBST	11,368	2	3	39.00	-	-	-
SL4459	ShpAST	11,289	2	2	22.11	-	-	-
SLP2_0003	RelB4	10,176	1	1	7.87	-	-	-
SLP2_0004	RelE4ST	10,888	1	4	15.05	-	-	-
PSLT028	CcdBST	11,581	3	13	40.59	3	8	40.59
PSLT107	VapB2ST	8,636	1	2	10.53	2	3	22.37
PSLT106	VapC2ST	14,879	2	6	21.21	3	7	29.55

(*) Subscript ST is added for clarification purposes to refer exclusively to TA protein characterized functionally in S. Typhimurium (see Fig. 2). Toxins are underlined.

(†) Protein extracts prepared from bacteria grown in LB medium to stationary phase at 37°C in non-shaking conditions. Two independent experiments were performed. PSM, peptide spectrum match.

(‡) Protein extracts were prepared from non-growing intracellular bacteria at 24 h post-infection of BJ5ta human fibroblasts. Three independent experiments were performed. PSM, peptide spectrum match.