Table 2. Toxins and antitoxins of S. Typhimurium TA modules detected by mass spectrometry in bacteria isolated from fibroblasts and in bacteria grown in LB medium.
Extracellular (†) | Intracellular (‡) | |||||||
---|---|---|---|---|---|---|---|---|
Accesion Number | Protein (*) | Mass (Da) | Unique peptides | PSMs | Coverage (%) | Unique peptides | PSMs | Coverage (%) |
SL1479 | RelEST | 11,070 | 3 | 8 | 29.79 | 1 | 4 | 9.57 |
SL1480 | RelBST | 9,086 | 1 | 2 | 23.17 | - | - | - |
SL2379 | A1ST | 13,536 | 4 | 19 | 33.33 | - | - | - |
SL2380 | T1ST | 14,966 | 4 | 6 | 34.62 | - | - | - |
SL2658 | PasIST | 10,769 | 3 | 6 | 31.25 | - | - | - |
SL2659 | PasTST | 17,725 | 2 | 3 | 13.29 | - | - | - |
SL2884 | A2ST | 10,673 | 2 | 5 | 28.13 | 2 | 2 | 31.25 |
SL2885 | T2ST | 19,059 | 2 | 4 | 13.14 | 2 | 4 | 20.00 |
SL2936 | ParDST | 10,369 | - | - | - | 1 | 1 | 7.69 |
SL3011 | VapCST | 14,930 | - | - | - | 1 | 1 | 21.21 |
SL3012 | VapBST | 14,276 | 2 | 3 | 13.60 | 1 | 2 | 6.40 |
SL3437 | T3ST | 22,767 | 11 | 27 | 68.00 | 8 | 27 | 47.00 |
SL3484 | DinJST | 9,442 | 2 | 4 | 24.42 | - | - | - |
SL3524 | DocST | 13,580 | 1 | 2 | 7.38 | 1 | 2 | 10.66 |
SL3617 | T4ST | 17,693 | 2 | 5 | 22.36 | 1 | 3 | 8.07 |
SL3744 | RelB3ST | 11,754 | 4 | 6 | 36.45 | - | - | - |
SL3976 | SehBST | 15,915 | 1 | 2 | 7.75 | - | - | - |
SL3979 | SehCST | 12,326 | 1 | 1 | 8.33 | - | - | - |
SL4253 | A5ST | 10,972 | 1 | 2 | 9.28 | 2 | 4 | 23.71 |
SL4254 | T5ST | 17,636 | 1 | 1 | 7.98 | 1 | 6 | 7.98 |
SL4460 | ShpBST | 11,368 | 2 | 3 | 39.00 | - | - | - |
SL4459 | ShpAST | 11,289 | 2 | 2 | 22.11 | - | - | - |
SLP2_0003 | RelB4 | 10,176 | 1 | 1 | 7.87 | - | - | - |
SLP2_0004 | RelE4ST | 10,888 | 1 | 4 | 15.05 | - | - | - |
PSLT028 | CcdBST | 11,581 | 3 | 13 | 40.59 | 3 | 8 | 40.59 |
PSLT107 | VapB2ST | 8,636 | 1 | 2 | 10.53 | 2 | 3 | 22.37 |
PSLT106 | VapC2ST | 14,879 | 2 | 6 | 21.21 | 3 | 7 | 29.55 |
(*) Subscript ST is added for clarification purposes to refer exclusively to TA protein characterized functionally in S. Typhimurium (see Fig. 2). Toxins are underlined.
(†) Protein extracts prepared from bacteria grown in LB medium to stationary phase at 37°C in non-shaking conditions. Two independent experiments were performed. PSM, peptide spectrum match.
(‡) Protein extracts were prepared from non-growing intracellular bacteria at 24 h post-infection of BJ5ta human fibroblasts. Three independent experiments were performed. PSM, peptide spectrum match.