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. 2015 Mar 20;5:9356. doi: 10.1038/srep09356

Figure 1. Overview of miRFLP assay.

Figure 1

(a) The mixture of miRNAs and iRCSs is subjected to hybridization in solution with sequence-specific omega primers. (b) RT is performed. (c) After RNA removal, the newly extended portions of matched omega primers are used as probes in hybridization with template adapters. The hybrids are extended by DNA polymerase using adapter sequences as templates. (d) Competitive PCR amplification of correctly assembled fragments. (e) DNA fragment length analysis is performed by automated capillary electrophoresis.