Table 1. The UBC cell line secretome analysis.
| Cell line | Grade | No. of human peptides | No. of human proteins | No. of EGFR peptides | Secretome (EGFR) ng mg−1 | Cellular (EGFR) ng mg−1 | % Sec |
|---|---|---|---|---|---|---|---|
| UROtsa | NA | 5499 | 763 | 0 | 0.40 (0.03), 0.72 (0.08) | 43.7 (1.3), 61.4 (3.7) | 1.0 |
| MGH-U3 | 1 | 10 954 | 1041 | 5 | 4.29 (0.92), 4.46 (1.19) | 90.8 (4.7), 94.7 (8.9) | 4.7 |
| R-T4 | 1 | 8145 | 1160 | 1 | 1.30 (0.36), 2.62 (0.29) | 34.0 (18.0), 22.1 (11.8) | 9.6 |
| SW-780 | 1 | 4181 | 627 | 1 | 2.81 (0.32), 4.11 (0.62) | 49.3 (4.50), 62.2 (16.7) | 5.9 |
| RT112 | 2 | 5900 | 787 | 4 | 0.66 (0.07), 1.18 (0.17) | 82.4 (3.3), 62.0 (5.6) | 1.3 |
| VM-CUB-1 | 2 | 5915 | 765 | 16 | 10.8 (2.39), 9.40 (1.18) | 227 (3.79), 151 (41.7) | 5.5 |
| 5637 | 2 | 4188 | 628 | 3 | 0.41 (0.03), 0.68 (0.04) | 102 (3.81), 72.8 (0.87) | 0.7 |
| T-24 | 3 | 7087 | 916 | 0 | 1.19 (0.32), 2.37 (0.57) | 27.1 (1.36), 29.0 (3.18) | 6.3 |
| HB-CLS-2 | 3 | 7135 | 847 | 53 | 252 (92.8), 298 (22.3) | 2720 (161), 2646 (927) | 10.3 |
Abbreviations: EGFR=epidermal growth factor receptor; NA=not applicable; % Sec=percentage of secreted EGFR; UBC=urothelial bladder cancer.
The number of unique peptides identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in each secretome and the resulting protein identifications are shown alongside the number of EGFR peptides identified. Proteomic analysis was performed once per cell line. The concentration of EGFR (determined by ELISA on biological triplicates) in paired secretomes and cell lysates is shown as ng EGFR per mg of cellular protein (determined by bicinchoninic acid assay); left value= −PMA, right value= +PMA. The percentage of secreted EGFR (% Sec) is relative to cellular EGFR.