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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Biochem J. 2011 Dec 1;440(2):293–300. doi: 10.1042/BJ20100991

Figure 1. Substrate specificity and directionality of hSuv3.

Figure 1

(A) SDS/PAGE of purified hSuv3-(47–722)-WT and -K213A mutant, 500 ng per lane, 4–15% gel, silver stained. (B) Helicase assay performed as described in the Materials and methods section with D49/D50-2* forked substrate. WT and K213A hSuv3-(47–722) were used at 20 nM concentration. Energy cofactors used are indicated. Triangle denotes heat-denatured substrate. (C) Helicase assays with indicated DNA substrates. hSuv3-(47–722)-WT was used at the indicated concentrations.