Skip to main content
. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Biochem J. 2011 Dec 1;440(2):293–300. doi: 10.1042/BJ20100991

Figure 3. Inhibition of hSuv3 helicase activity by RPA.

Figure 3

(A) Unwinding activity of hSuv3 on a forked substrate in the presence of RPA. Purified recombinant proteins were mixed (50 nM hSuv3 and RPA as indicated) and the reaction was started by addition of the radioactively labelled DNA substrate (0.5 nM), followed by incubation at 37°C for 30 min. (B) Quantification from (A). hSuv3 helicase activity in the presence of RPA relative to helicase activity by hSuv3 alone. Products from RPA alone were subtracted from helicase activity values. Error bars represent S.D. (mean value for three experiments). *P < 0.001 in Student’s t test for values different from hSuv3 alone; **P < 0.0001. (C) Unwinding activity of hSuv3 in the presence of mtSSB as in (A). (D) Quantification of fold stimulation from (C). Error bars represent S.D. (mean value for three experiments).