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. Author manuscript; available in PMC: 2015 Mar 20.
Published in final edited form as: Biochem J. 2011 Dec 1;440(2):293–300. doi: 10.1042/BJ20100991

Figure 5. Stimulation of FEN1 incision activity by hSuv3.

Figure 5

(A) FEN1 incision on a long (15 nt) flap in the presence of hSuv3-WT, hSuv3-K213A or BSA. Purified recombinant proteins were mixed (1 nM FEN1) and the reaction started by addition of the radioactively labelled DNA substrate (0.5 nM), followed by incubation at 37°C for 15 min. (B) Quantification of fold stimulation from (A). Error bars represent S.D. (mean value for five experiments). (C) FEN1 incision on a short (1 nt) flap in the presence of hSuv3-WT, hSuv3-K213A or BSA. (D) Quantification of fold stimulation from (C). Error bars represent S.D. (mean value for four experiments). P values were determined using Student’s t test.