Table 1.
TaqMan assay design including primer and probe sequences
| Genes | Mutations | Forward primer | Reverse primer | VIC probe | FAM probe |
|---|---|---|---|---|---|
| DNAI1 | c.1490G>A | CCTCTTGGAACTGGGCTAAGC | CATGTAGTCAATCTCTTTGTGGAAGTCA | TCTTTTCCCAAGGTTGTG | TTTTCCCAAGATTGTG |
| DNAI2 | c.1304G>A | CGTTTTCTTTACCACCAGGATGGA | GGATCGCACTGCTCGAACAT | CTGGATATCTGGGACTTC | CCTGGATATCTAGGACTTC |
| DNAH11 | c.6244C>T | GTGCTATTAAGTCTGTCTTGGTTGTG | TCATATTAGCATTGCAGTACCTGATCTTC | ATTTTTATCTCCTCGTTTCAG | TTTTTATCTCCTCATTTCAG |
| DNAH11 | c.11929G>T | CAGGAGACGGTGGCAGAAG | CCCAGTGTCCTCCTTTGGAA | TGGCCCTGGAGAAAG | TGGCCCTGTAGAAAG |
| DNAH5 | c.6988+2T>C | GGGATATTTTCTACGCTTTGGAGGAA | GTATAGCCTCCAAGGATTCTATCTAGAAGT | TTGTAATTTATCTTCTACCTTTCT | AATTTATCTTCTGCCTTTCT |
| DNAH5 | c.7502G>C | TCGCGCTGCTGTGGAG | CGCAGCCAGAGCTCCAG | ACGGACGGCGCCGC | ACGGACCGCGCCGC |
| DNAH5 | c.5545G>A | TGATATGGACACGGGATTCAGAAGA | CTCCAGGAAAGCCTGATTAGTTTTC | AGCCCTTAGAAATGCCAAGT | AGCCCTTAGAAATACCAAGT |
| CCDC40 | c.248delC | AAGCGGAAGCTGCAATTGA | TCCTCTTCGCTTTCAGCATCTC | AGGAGGCTGTGTCCTA | AGGAGGTGTGTCCTATG |
| CCDC65 | c.877_878delAT | GCCATAACTATTTCAAAAGGCAAGATCA | CGCAGTTGTACAAGGACCAATTC | ATGAGAACCGGTATATCCGTA | ATGAGAACCGGTATCCGTA |
| CCDC114 | c.939delT | TCATCAACGAGCAGAACTTGGA | AGGCCTCACTCACCTCCTTGA | CTGGAGCATGTGCAGGA | AGCAGTGCAGGAAGA |
| C21orf59 | c.735C>G | GAGGAGCAGAAGCAGCTGAT | GCACTGCAGAAAGCCCATCT | TGTCTTCTGTGATAGTACAGC | TGTCTTCTGTGATACTACAGC |
GenBank reference sequence and version number for the genes studied: DNAI1 (NM_012144.2), DNAI2 (NM_023036.4), DNAH11 (NM_001277115.1), DNAH5 (NM_001369.2), CCDC40 (NM_017950.3), CCDC65 (NM_033124.4), CCDC114 (NM_144577.3), and C21orf59 (NM_021254.2).