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. 2015 Mar 10;180(1):155–163. doi: 10.1111/cei.12565

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© 2014 The Authors. Clinical and Experimental Immunology published by John Wiley & Sons Ltd on behalf of British Society for Immunology.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Detecting serum immunoglobulin (Ig)G autoantibodies that bind to constituents of human lung tissue. Sera from smokers with chronic obstructive pulmonary disease (CS), smokers (S) and non-smokers (NS) were analysed by enzyme-linked immunosorbent assay (ELISA) for IgG antibodies that bind to human lung tissue homogenates. The sources of the lung tissue were as follows: (a,b) CS patients; (c,d) S subjects; (e,f) NS subjects. Each point represents an individual serum sample, and the horizontal line is the median optical density (OD) value of the group; statistical comparisons between groups were performed using the Mann–Whitney U-test. The lung samples and the sera were obtained from different subjects.