Table 2.
Candidate antigens – the source, the concentration used and the manufacturing company.
| Antigen | Source | Concentration μg/ml | Company |
|---|---|---|---|
| Collagen-1 | Human lung | 10 | Sigma, Aldrich, Poole, UK |
| Collagen-2 | Human cartilage | 1 | Chemicon International, Watford, UK |
| Collagen-3 | Human placenta | 10 | Sigma, Aldrich, Poole, UK |
| Collagen-4 | Human placenta | 10 | Sigma, Aldrich, Poole, UK |
| Collagen-5 | Human placenta | 10 | Sigma, Aldrich, Poole, UK |
| Fibronectin | Human plasma | 10 | Sigma, Aldrich, Poole, UK |
| Laminin | Human plasma | 10 | Sigma, Aldrich, Poole, UK |
| Elastin peptides | Human lung elastin peptides | 25 | Elastin product company, Owensville, MO, USA. |
| Vitronectin | Human plasma | 1 | Sigma, Aldrich, Poole, UK |
| Vimentin | Human recombinant protein | 1 | Progen Biotechnik, Heidelberg, Germany |
| Cytokeratin-8 | Human recombinant protein | 1 | GenWay Biotech, San Diego, CA, USA |
| Cytokeratin-18 | Human recombinant protein | 1 | GenWay Biotech, San Diego, CA, USA |
| Thyroglobulin | Purified human | 10 | Sigma, Aldrich, Poole, UK |
| Bovine serum albumin (BSA) | Bovine serum | 10 000 | Sigma, Aldrich, Poole, UK |