Fig. 1. Detection limit of the SD MPT64 Rapid test for Mycobacterium (M.) bovis. The test was used to analyze a series of diluted suspensions of M. bovis AN5 cultured in Middlebrook 7H9 broth with enrichment. A representative image of three tests is shown. The results were interpreted as positive (+), negative (-), or weak (±). NC, negative control; 1, 2.1 × 103 CFU/mL of M. bovis; 2, 4.2 × 103 CFU/mL of M. bovis; 3, 8.5 × 103 CFU/mL of M. bovis; 4, 1.7 × 104 CFU/mL of M. bovis; 5, 3.4 × 104 CFU/mL of M. bovis; 6, 6.8 × 104 CFU/mL of M. bovis; 7, 1.3 × 105 CFU/mL of M. bovis; 8, 2.7 × 105 CFU/mL of M. bovis; 9, 5.4 × 105 CFU/mL of M. bovis; 10, 1.0 × 106 CFU/mL of M. bovis; 11, 2.0 × 106 CFU/mL of M. bovis.
