Fig. 2. Phylogeneticanalysis of TLR5. Aneighbor-joining (NJ) tree was constructed using MegAlign. The sequences were derived from the predicted amino acidsequences of thePekin duck TLR5 (KF717594) and GenBank entries for the domestic goose (Anser anser, accession no. AEP71332.1), chicken (Gallus gallus domesticus, accession number. ACR26276.1), cattle (Bos Taurus, accession no. AFR42399.1), chimpanzee (Pan troglodytes, accession no. NP_001123934.1), cat (Felis catus, accession no. XP_004001379.1), gray wolf (Canis lupus, accession no. NP_001184105.1), human (Homo sapiens, accession no. AAI09119.1), macaque (Maca camulatta, accession no. NP_001123901.1), mouse (Mus musculus, accession no. NP_058624.2), pig (Sus scrofa, accession no. AGT79978.1), rabbit (Oryctolagus cuniculus, accession no. AEA11027.1), rat (Rattus norvegicus, accession no. NP_001139300.1), sheep (Ovis aries, accession no. NP_001129398.1), turkey (Meleagris gallopavo, accession no. ADX33343.1), water buffalo (Bubalus bubalis, accession no. AEY63776.1), zebra finch (Taeniopygia guttata, accession no. XP_002188762.1), carp (Cyprinus carpio, accession no. AGH15501.1), fugu rubripes (Takifugu rubripes, accession no. AAW69374.1), zebrafish (Danio rerio, accession no. NP_001124067.1), rattlesnake (Crotalus adamanteus, accession no.AFJ51724.1), American bison (Bison bison, accession no. AEY63777.1), chamois (Rupicapra rupicapra, accession no. AFR42404.1), and deer (Odocoileus virginianus, accession no. AFR42402.1). The phylogenetic tree was generated with Clustal X software using the NJ method.