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. 2015 Feb 20;194(7):3236–3245. doi: 10.4049/jimmunol.1402764

FIGURE 3.

FIGURE 3.

The type I IFN response to cytosolic F. novicida requires cGAS, Ifi204, and STING in RAW264.7 macrophages. Type I IFN levels were measured from RAW264.7 (WT) macrophages and the indicated genotypes (A, C, and E) infected with F. novicida strains at an MOI of 10 for 24 h or not infected (NI). Type I IFN levels were measured from deficient macrophages stably expressing Sting (B), cGAS (D), or Ifi204 (F) infected with U112 at an MOI of 10 for 24 h. Type I IFN levels were measured from RAW264.7 (WT) macrophages and indicated genotypes infected with F. novicida for 12 (G) and 24 h (H) or infected with WT L. monocytogenes for 8 h at an MOI of 20 (I). mRNA expression levels of IFN-β1 (J) and TNF-α (K) from uninfected and U112-infected macrophage cell lines at an MOI of 10 for 8 h. mRNA levels were normalized to GAPDH and the WT RAW264.7 macrophages. (L) Type I IFN levels were measured from macrophages stimulated with LPS for 8 h. Graphs show the mean ± SD of triplicate wells and are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001. NS, not stimulated.