FIGURE 4.

Both cGAS and Ifi204 are required for the full type I IFN response to F. novicida infection. mRNA expression levels were measured in uninfected RAW264.7 (WT) cell lines stably expressing Ifi204 (A) or cGAS (C). mRNA expression was normalized to GAPDH and WT RAW264.7 macrophages. Macrophages were infected with WT F. novicida at an MOI of 10 for 24 h and type I IFN levels measured (B and D, respectively). Graphs show the mean ± SD of triplicate wells and are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.