FIGURE 5.

cGAS and Ifi204 cooperate to sense cytosolic dsDNA. (A) Type I IFN levels were measured from WT RAW264.7 macrophages transfected for 24 h with U112 lysates either untreated (UT) or treated with DNase I, heat-killed (HK) DNase I or RNase A. Type I IFN levels were measured from RAW264.7 macrophages transfected for 24 h with (B) endo-free plasmid DNA (pCherry), (C) 1 μg/ml polyinosinic-polycytidylic acid [poly(I:C)], or (D) 10 μg/ml c-di-GMP NT, not transfected. Graphs show the mean ± SD of triplicate wells and are representative of three independent experiments. ***p < 0.001.