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. 2015 Feb 20;194(7):3236–3245. doi: 10.4049/jimmunol.1402764

FIGURE 6.

FIGURE 6.

cGAS increases inflammasome activity in BMMs. (A) Cytotoxicity, (B) IL-1β secretion, and (C) intracellular survival were measured from unstimulated primary C57BL/6 (WT), Stinggt/gt, cGAS−/−, Aim2−/−, and ASC−/− BMMs infected with F. novicida strains at an MOI of 10 for 12 h unless otherwise indicated. (D) Release of processed caspase-1 (casp-1 p10 and p20) into the supernatants (SN) was measured by Western blot from cells either not infected (NI) or infected with WT F. novicida at an MOI of 100 for 12 h. Corresponding cell lysates were probed for procaspase-1 and β-actin. Graphs show the mean ± SD of triplicate wells and are representative of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.