FIGURE 1.
OSCAR–collagen signaling promotes DC survival. Human monocytes were purified and differentiated into iDCs by GM-CSF and IL-4 (50 ng/ml each) for 6 d. Then, 1 × 106 iDCs/ml were cultured on either uncoated or ColII-coated plates. To the ColII-coated wells, OSCAR-blocking Ab (1 μg/ml) or control Ab (1 μg/ml) was added. GM-CSF (50 ng/ml) was added as a positive control of survival. As a negative control, iDCs were left in growth factor–free medium. After 7 d, cell viability was assessed using alamarBlue. Data are expressed as mean of biological triplicates ± SD. Data shown are from one representative donor out of five analyzed.