Table II. Cytokines and chemokines secreted by DCs.
| iDCs | Col-DCs | Col-DCs + Anti-OSCAR | Col-DCs + Isotype mAb | LPS-DCs | |
|---|---|---|---|---|---|
| IL-6 | 2,428.0 ± 1.5 | 8,565.0 ± 175.8* | 1,729.0 ± 97.7** | 6,750.0 ± 501.1ns | 22,547.0 ± 364.4** |
| IL-8 | 11,406.0 ± 485.5 | 20,167.0 ± 523.4** | 11,809.0 ± 457.6** | 19,911.0 ± 699 ns | 20,286.0 ± 508.0** |
| IL-10 | 239.5 ± 48.4 | 2,801.0 ± 188.0* | 85.5 ± 11.0* | 2,330.0 ± 48.4ns | 12,049.0 ± 126.4** |
| IL-12p40 | 536.2 ± 35.0 | 46,361.0 ± 406.0** | 595.6 ± 49.0** | 46,937.0 ± 2,298.0ns | 39,407.0 ± 1771.0* |
| IL-12p70 | 71.5 ± 33.7 | 99.7 ± 6.1ns | 40.1 ± 1.5* | 86.7 ± 0.0ns | 14,287.0 ± 315.8** |
| IL-13 | 21.2 ± 11.2 | 1,034.0 ± 34.3* | 11.5 ± 0.0* | 1,039.0 ± 34.7ns | 1,658.0 ± 6.9** |
| RANTES | 64.0 ± 15.4 | 2,201.0 ± 132.1* | 54.8 ± 0.5* | 2,131.0 ± 52.9ns | 44,79.0 ± 193.4* |
| TNF-α | 366.6 ± 101.2 | 44,763.0 ± 1881.0* | 78.1 ± 0.0* | 28,719.0 ± 2669.0ns | 31,108.0 ± 1961.0* |
| IL-23 | 0.4 ± 0.4 | 272.2 ± 6.5*** | 0.8 ± 3.4*** | 289.3 ± 40.1ns | 696.3 ± 8.6*** |
Multiple cytokines were measured in the supernatants from DCs cultured at the indicated conditions using Luminex technology. Values are shown in pg/ml. Data represent means ± SD of triplicate wells treated independently. Data are shown for 1 representative donor out of >20 donors analyzed with similar results. IL-23 was measured using a sandwich ELISA.
ns
nonsignificant.
*
p < 0.05, **p < 0.01, ***p < 0.001 by t test for the following conditions: iDCs versus Col-DCs and for Col-DCs versus Col-DCs plus anti-OSCAR.
LPS-DCs, LPS-matured DCs.