Table III. Cytokines and chemokines secreted by T cells cocultured with allogeneic DCs.
| iDCs | Col-DCs | Col-DCs + Anti-OSCAR | Col-DCs + Isotype mAb | |
|---|---|---|---|---|
| IL-2 | 38.4 ± 13.2 | 3,135.0 ± 463.0** | 35.8 ± 18.0** | 2,666.0 ± 543.0ns |
| IL-3 | 811.8 ± 16.4 | 771.7 ± 6.1* | 782.3 ± 62.0ns | 751.7 ± 65.5ns |
| IL-5 | 1.4 ± 0.2 | 2,087.0 ± 234.0** | 1.3 ± 0.0** | 1,919.0 ± 598.9ns |
| IL-6 | 12.0 ± 3.7 | 1,414.0 ± 329.5* | 20.0 ± 10.8* | 1,569.0 ± 163.7ns |
| IL-8 | 1,035.0 ± 761.8 | 10,852.0 ± 859.8** | 1,352.0 ± 458.4** | 10,936.0 ± 274.1ns |
| IL-10 | 13.9 ± 1.4 | 99.0 ± 65.2ns | 14.2 ± 2.5ns | 147.2 ± 54.8ns |
| IL-12p40 | 1,339.0 ± 65.4 | 10,083.0 ± 292.1** | 1,349.0 ± 56.5*** | 13,601.0 ± 403.8ns |
| IL-12p70 | 10.4 ± 0.0 | 13.0 ± 0.0ns | 11.3 ± 1.5ns | 14.3 ± 1.3ns |
| IL-13 | 2.5 ± 0.8 | 3,547.0 ± 142.3*** | 2.5 ± 0.2*** | 3,609.0 ± 321.5ns |
| IL-17 | 46.1 ± 3.2 | 48.9 ± 3.2ns | 48.9 ± 3.2ns | 49.4 ± 5.6ns |
| IL-18 | 25.8 ± 2.5 | 25.2 ± 1.4ns | 24.2 ± 0.7ns | 26.4 ± 1.6ns |
| GM-CSF | 26.1 ± 3.9 | 1,402.0 ± 217.4** | 23.9 ± 2.5** | 1,581.0 ± 135.2ns |
| IFN-γ | 24.5 ± 2.0 | 254.9 ± 69.4* | 23.1 ± 2.4* | 514.0 ± 195.1ns |
| MCP-1 | 992.4 ± 549.3 | 85.6 ± 2.3ns | 1,505.0 ± 654.3ns | 86.0 ± 8.8ns |
| M-CSF | 268.5 ± 12.0 | 347.6 ± 20.0* | 248.4 ± 6.4** | 357.1 ± 30.7ns |
| RANTES | 14.6 ± 6.3 | 2,180.0 ± 230.4** | 18.4 ± 9.6** | 2,287.0 ± 152.2ns |
| TNF-α | 104.3 ± 56.4 | 3,830.0 ± 168.4*** | 101.9 ± 18.1*** | 3,575.0 ± 567.1ns |
Multiple cytokines were measured in the supernatants of T cells cultured with the indicated allogeneic DCs. Supernatants were collected at day 4 of coculture. Anti-OSCAR mAbs or isotype control mAbs were used at 1 μg/ml. Values are shown in pg/ml. Data represent means ± SD of triplicate wells treated independently. Data are shown for one representative experiment out of four independent experiments with similar results.
ns
nonsignificant.
p < 0.05, **p < 0.01, ***p < 0.001 by t test for the following conditions: iDCs versus Col-DCs, Col-DCs versus Col-DCs + anti-OSCAR, and Col-DCs versus Col-DCs + isotype.