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. 2015 Mar 20;6:173. doi: 10.3389/fpls.2015.00173

Figure 1.

Figure 1

(A) Bicellular pollen is exemplified by Nicotiana benthamiana, and (B) tricellular pollen by Oryza sativa (rice). Both of these anthesis pollen grains are labeled with DAPI, captured as a MIP using confocal laser scanning microscopy, and manually-segmented to portray generative and sperm nuclei in cyan, vegetative nucleus in green and pollen wall autofluorescence in beige. Scale bars = 5 μm.