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. 2015 Mar 20;7:32. doi: 10.3389/fnagi.2015.00032

Figure 4.

Figure 4

Administration of TGF-β1 inhibitors after noise exposure. (A) Representative ABR waveforms in response to click stimulus in C57 exposed to VS2–20 noise at 110 dB SPL for 30 min and then treated with TGF-β1 inhibitor P17 at 2.5 mg/kg/12 h or saline for 15 days. Bold lines indicate the ABR threshold. (B) Evolution of click ABR thresholds (mean ± SEM, in dB SPL) in C57 mice exposed to VS2–20 noise at 110 dB SPL for 30 min and then treated with TGF-β1 inhibitor P17 (triangles) at 2.5 mg/kg or saline (circles) at 0.1 ml/10 g once (white symbols) or twice (black symbols) daily for 15 days (n = 6 per group). Statistically significant differences (paired T test, **p ≤ 0.01) were found between mice treated with P17 at 2.5 mg/kg/12 h and saline treated mice. Similar results were observed with P144 (data not shown). (C) Audiogram of CBA mice treated with P144 (black squares) at 10 mg/kg/day for 15 days after noise exposure (VS2–20 at 110 dB SPL, 30 min) showed statistically significant differences (paired T test, *p ≤ 0.05, ***p ≤ 0.001) at the end of the treatment compared to saline treated mice (black circles). Non-exposed mice (white circles) maintained normal hearing thresholds throughout the study. Similar results were observed with P17 treatment (data not shown).