Skip to main content
. 2015 Apr 1;22(10):819–831. doi: 10.1089/ars.2014.6017

FIG. 1.

FIG. 1.

Effects of Nrf1 silencing on intracellular levels of GSH and ROS and insulin secretion in MIN6 β-cells. (A) mRNA expression of Nrf1 determined by real-time RT-qPCR. The primer set detects isoform-313, −453, −572, −583, −741, and −742 of Nrf1. Scramble, cells transduced with nontarget negative control shRNA; Nrf1-KD, cells with knockdown of Nrf1. n=9–12. *p<0.05 versus Scramble. (B) Intracellular levels of GSH. n=9. *p<0.05 versus Scramble. (C) Intracellular ROS levels. n=11. *p<0.05 versus Scramble. (D) mRNA expression of proinsulin. n=6–9. (E) Insulin content. n=13–36. (F) Insulin secretion. Glu, glucose; KCl, potassium chloride; ratio, insulin secreted under 20 mM over 3 mM Glu. n=19–21. *p<0.05 versus the same cell types with 3 mM Glu; #p<0.05 versus Scramble with the same treatment. Nrf1, nuclear factor-erythroid 2-related factor 1; ROS, reactive oxygen species; RT-qPCR, reverse transcription-quantitative PCR.