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. 2015 Apr 1;22(10):819–831. doi: 10.1089/ars.2014.6017

FIG. 3.

FIG. 3.

Expression and secretion of insulin in Nrf1(b)-KO mice. Animal age is 11–17 weeks. (A) In vivo analysis of GSIS in Nrf1(b)-KO and Nrf1(b)-WT mice. Basal, fasting plasma insulin levels; Glu-stimulated, plasma insulin levels post glucose challenge (1.0 mg of glucose/g of BW; IP injection, 15 min). n=7–20. *p<0.05 versus Basal of the same genotype; #p<0.05 versus Nrf1(b)-WT under basal condition. (B) In vivo analysis of GSIS in Nrf2(b)-KO and Nrf2(b)-WT mice. Measurements were conducted under the same conditions as in (A). n=6–16. (C) mRNA expression of proinsulin in cultured islets. Isolated islets were cultured for 48 h before mRNA isolation. n=5–6. (D) Insulin content in islets after a 48-h culture. n=14. (E) GSIS in cultured islets. The levels of secreted insulin in 30 min were normalized by DNA content. LG, low glucose (3 mM); HG, high glucose (16.7 mM); KCl, 30 mM potassium chloride. n=4–12. *p<0.05 versus LG of the same genotype; #p<0.05 versus Nrf1(b)-WT with LG. GSIS, glucose-stimulated insulin secretion.