FIG. 6.

Effects of Hk1 silencing on glycolysis and insulin secretion in Nrf1-KD MIN6 cells. (A) Protein expression of HK1 and glycolysis-related proteins. Nrf1-KD:Scramble, Nrf1-KD cells transduced with scramble shRNA; Nrf1-KD:Hk1-KD, Nrf1-KD cells transduced with shRNA against Hk1. n=3. (B) Representative image of pH changes of culture media. Confluent cells were cultured for 48 h. (C) ECAR measured in Kreb's buffer with 3 mM glucose followed by addition of 20 mM glucose (HG). n=3–4. (D) Insulin secretion. Glu, glucose. n=4–6. *p<0.05 versus 3 mM Glu of Nrf1-KD:Hk1-KD cells; ^#p<0.05 versus Nrf1-KD:Scramble cells with 3 mM Glu. (E) Glycolytic inhibition suppresses basal insulin release in MIN6 cells. Scramble and Nrf1-KD MIN6 cells were incubated with 2-DG in Kreb's buffer with 3 mM Glu. n=4. *p<0.05 versus the same cells with 3 mM Glu; ^#p<0.05 versus Scramble with the same treatment. HK1, hexokinase 1. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars