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. 2015 Feb 22;26(3):161–171. doi: 10.1089/hum.2014.050

FIG. 2.

FIG. 2.

Synovial fluid concentration and mRNA expression of IGF-I from rAAV2 and rAAV5 vectors after direct intraarticular (IA) injection. (A) IGF-I concentration (ng/ml) in the synovial fluid over time in joints injected with either rAAV2-IGF-I, rAAV5-IGF-I, or DPBS. IGF-I concentration was measured by ELISA. Graphs illustrate mean values±SEM averaged from four horses per group. *IGF-I concentration significantly increased in rAAV5- and rAAV2-injected horses compared with DPBS-injected horses (p<0.05). (B) IGF-I mRNA expression in the synovial membrane 56 days after IA injection of either rAAV2-IGF-I, rAAV5-IGF-I, or DPBS. IGF-I values were transformed, using natural log transformation. mRNA was measured by qPCR and expressed as total copy number, using a validated standard curve. *p<0.05.