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. 2015 Apr 1;22(10):848–870. doi: 10.1089/ars.2014.5868

FIG. 5.

FIG. 5.

TXNIP mediates fructose-induced NLRP3 inflammasome activation and lipid metabolism-related gene deregulation in cultured hepatocytes. (A) Primary rat hepatocytes, RHPCs, HLO2, and HepG2 cells were transfected with or without TXNIP siRNA for 48 h. TXNIP expression levels were analyzed by RT-PCR (n=4/group). (B) Primary rat hepatocytes, RHPCs, HLO2, and HepG2 cells were transfected with TXNIP siRNA for 48 h and co-incubated with or without 5 mM fructose for another 72 h. NLRP3, ASC, and caspase-1 protein levels were quantitated by Western blot (n=8/group). (C) IL-1β and IL-18 concentrations in cell lysate (top) and supernatant (bottom) were determined by ELISA (n=8/group). (D) Western blot analysis of PPAR-α, SREBP1, and SCD1 protein levels (n=8/group). (E) Primary rat hepatocytes and RHPCs were transfected with NLRP3 siRNA for 48 h and co-incubated with or without 5 mM fructose for another 72 h. TXNIP protein levels were measured by Western blot (n=8/group). (F) Primary rat hepatocytes and RHPCs were co-incubated with or without 5 mM fructose and the caspase-1 specific inhibitor Ac-YVAD-CMK for 72 h, after which TXNIP protein levels were analyzed by Western blot (n=8/group). Results are expressed as mean±SEM. #p<0.05, ##p<0.01, ###p<0.001 versus control group.