FIG. 4.
PCR evaluation of fetal dissemination of adenoviral vector after placental gene transfer. PCR for the adenoviral E4 region of the viral vectors was performed in various fetal organs of fetuses whose placentas were injected with 1×109 PFU of Ad-LacZ. No adenoviral DNA was found in the fetal liver, kidney, gonad, or heart. Some adenovirus was detected in the fetal lung. Intense bands were detected in the placenta from both maternal and fetal sides. Water sample served as a negative control and adenovirus E4 was included as a positive control.