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. 2015 Jan 20;290(12):7369–7387. doi: 10.1074/jbc.M114.626747

TABLE 2.

Bacterial signal peptides as FPR agonists

Listed is a set of nine bacterial signal peptides that we initially identified, their sources, sequences, accession numbers (UniProt database), and mean EC50 values (in nm) with respect to the activation of heterologously expressed hFPR1, mFpr1, hFPR2, mFpr2, hFPR3, and mFpr-rs1. EC50 values were obtained by measuring full concentration-response curves. Number of experiments (n) is given in parentheses. n.d., no data; n.r., no response with the highest tested concentration (30 μm).

Signal peptide Source Species Symptoms Monocytes hFPR1 mFpr1 hFPR2 mFpr2 hFPR3 mFpr-rs1
Streptococcus-SP1 Hyaluronidase (Q8VLQ8) Streptococcus suis Meningitis, sepsis, skin lesions 6.4 ± 7.7 (13) 1.8 ± 1.6 (4) 2.2 ± 0.09 (3) 1343 ± 614 (4) 1013 ± 84 (3) n.r (4) n.r (3)
Streptococcus-SP1-full Hyaluronidase (Q8VLQ8) Streptococcus suis Meningitis, sepsis, skin lesions 24 ± 17 (4) 30 ± 29 (2) n.d. 54 ± 35 (2) n.d. n.d. n.d.
Bacillus-SP2 Hemolysin (Q45105) Bacillus cereus Food poisoning n.d. 12850 (1) 1981 ± 732 (3) 568 (1) 78 ± 34 (3) n.r (1) n.r (3)
Staphylococcus-SP3 Cell wall hydrolase LytN (Q6GHI8) Staphylococcus aureus Abscess, cellulitis, pneumonia 164 ± 134 (3) 61 ± 31 (3) 26 ± 15 (3) 293 ± 143 (3) 437 ± 155 (3) n.r (3) n.r (3)
Salmonella-SP4 Membrane protein YaeT (Q8Z9A3) Salmonella typhimurium Enteric fever n.d. 11520 ± 1888 (2) 6862 ± 3985 (3) 31 ± 16 (2) 417 ± 318 (4) n.r (2) n.r (4)
Haemophilus-SP5 Tail-specific protease (P45306) Haemophilus influenzae Fever, meningitis, bronchitis 232 ± 97 (5) 705 ± 238 (2) 100 ± 48 (3) 8 ± 2.0 (2) 62 ± 60 (3) n.r (2) n.r (3)
Psychromonas-SP6 Phosphatetransferase (A1ST24) Psychromonas ingrahamii n.d. 0.19 ± 0.14 (3) 0.15 ± 0.06 (4) 0.07 ± 0.03 (4) 38 ± 34 (4) 93 ± 58 (4) >10000 (4) 284 ± 79 (4)
Shewanella-SP7 Siderophore receptor (A6WUI7) Shewanella baltica n.d. 64 ± 32 (3) 7.7 ± 4.5 (3) 2.4 ± 3.1 (3) 6.7 ± 4.0 (3) 680 ± 920 (3) n.r (3) n.r (3)
Desulfotomaculum-SP8 Membrane protein (A4J7E6) Desulfotomaculum reducens n.d. 0.09 ± 0.04 (7) 0.25 ± 0.17 (4) 0.17 ± 0.26 (4) 34 ± 25 (4) 144 ± 226 (4) >10000 (4) 269 ± 245 (3)
Desulfotomaculum-SP8-full Membrane protein (A4J7E6) Desulfotomaculum reducens n.d. 735 ± 202 (4) 1090 ± 9 (2) n.d. 1512 ± 249 (2) n.d. n.d. n.d.
Borrelia-SP9 Basic membrane protein D (Q44743) Borrelia burgdorferi Lyme borreliosis n.d. 11379 ± 7930 (3) 5806 ± 3150 (4) 957 ± 640 (3) 1067 ± 584 (3) n.r (4) n.r (3)
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