FIGURE 9.

Exportin-1 function is required for the presence of cytosolic RNA:DNA hybrids. A, A549 cells were treated with the indicated concentrations of the exportin-1 inhibitor leptomycin B (LMB) for 16 h before staining with RNA:DNA hybrid-specific antibody S9.6 (green), COX IV (red), and Hoechst (blue). Scale bars = 10 μm. B, A549 cells were treated with increasing concentrations of leptomycin B (0, 10, 20, 30, and 40 nm) for 16 h before staining with RNA:DNA hybrid-specific antibody S9.6, COX IV, and Hoechst. Microscope images were quantified for cytosolic and mitochondrial intensity of cells. Student's two-tailed t test was performed. Error bars represent S.E. *, p < 0.05.