TABLE 1.
Theoretical and experimentally determined molecular weights for ligand-free and ligand-bound complexes
| Protein | Theoretical molecular mass | Mass spectrometry | Predicted molecular massa | SEC | Sedimentation velocity | SAXS |
|---|---|---|---|---|---|---|
| kDa | kDa | kDa | kDa | kDa | kDa | |
| Myostatin | 24.8 | 24.8 | NDb | NDb | NDb | |
| GASP-1 | 60 | 68.7 (62.2)c | 41 | 61.3 | 76.4 | |
| GASP-1·myostatin (1:1) | 84.8 | 93.5 | 114 | 97.1 | 103.6 | |
| GASP-1·myostatin (2:1) | 144.8 | 162.2 | ||||
| GASP-2 | 57 | 62.3 (58.1+) | 28 | 60.4 | 74.5 | |
| GASP-2·myostatin (1:1) | 81.8 | 87.1 | ||||
| GASP-2·myostatin (2:1) | 138.8 | 149.4 | 160 | 137 | 187.3 |
a Values indicate the predicted molecular mass based on the molecular mass determined by mass spectrometry.
b ND, not determined.
c Mass following treatment with endoglycosidases.