TABLE 3.

Experimentally determined parameters from SAXS analysis of ligand-free and ligand-bound GASP proteins

Sample	Concentration	I(0)	Rg		Dmax	Volume	Molecular mass	DAMMIF NSDa
			Gunier	Real space
	mg/ml	cm−1	Å	Å		Å3	Da	Å
GASP-1	2.6	682	53.1	53.85	177	347,796	86,000
	1.6	393	51.9	52.06	172	341,665	75,600	0.945 ± 0.06
	1	240	51	50.97	168	341,668	67,500
GASP-1·myostatin	1.4	396	49.1	48.12	163	432,793	112,000
	1.2	303	47.5	46.52	160	433,075	96,700	1.034 ± 0.057
	1	252	48.8	46.33	161	422,324	102,000
GASP-2	3	556	46.3	46.19	151	321,500	77,600
	2	369	45.6	43.51	147	326,803	71,300	0.828 ± 0.047
	1	191	44.8	41.19	141	316,598	61,000
GASP-2·myostatin	3	1130	62.6	64.83	205	595,832	211,000
	2	661	60.5	63.32	210	589,810	189,000	1.488 ± 0.478
	1	336	58.1	59.97	200	528,526	162,000
MBP GASP-1 WFIK·myostatin	3	357	56.7	56.5	173	376,242	179,000
	2	249	55.6	55.1	167	337,781	167,000
	1	127	52.7	54.5	168	349,291	174,000

^a NSD, normalized spatial discrepancy.