FIGURE 11.

Sequence analysis of YLR290C and related proteins. In A, YLR290C resides in a protein cluster populated by homologous fungal sequences including proteins that are fused to Coq10. Plant-like homologs form a distinct cluster composed of proteins from photosynthetic organisms. Prokaryotic sequences form a third independent cluster. The closest identified SDR subfamily distinct from YLR290C-like proteins includes human NDUFA9, a subunit of complex I, and orthologs in animals, fungi, and plants. In B, sequences representing each cluster from the protein similarity network in A were used to build a neighbor-joining tree. The tree is drawn to scale, with branch lengths in the same units (amino acid substitutions per site) as those of the evolutionary distances used to infer the phylogenetic tree. A branch length scale bar is shown below. A number after an organism name signifies the presence of multiple homologs in the tree. Protein identifications can be found in the supplemental Data Set 1. A schematic of the YLR290C-Coq10 fusion found in U. maydis is shown in C.