TABLE 2.
Residue substitutions of anti-HER2 × EGFR hetero-IgG1 variants in the presence of tags showed improved dual antigen binding and single band in Western blot
| Variant | Anti-HER2 HC1 (K392D + K409D) |
Anti-HER2 LC1 |
Anti-EGFR Fn3_HC2 (E356K + D399K) |
Anti-EGFR LC2_Fn3_FLAG_His6 |
||||
|---|---|---|---|---|---|---|---|---|
| VH1 | CH1 | VL1 | Cκ | VH2 | CH1 | VL2 | Cκ | |
| 1C02 | Q39K + Q105K | K147D + S183D | Q38D + A43D | S131K + S176K | G44D + Q105D | S183K | G100K + A43K | S131D + S176D |
| 1C04 | Q39K + Q105K | K147D + S183D | Q38D + A43D | S176K + T180K | G44D + Q105D | S183K | G100K + A43K | Q124D + S176D |
| 2A05 | Q39K + Q105K | K147D | Q38D + A43D | Q124K | G44D + Q105D | K147K | G100K + A43K | S131D |
| 2B05 | Q39K + Q105K | K147D | Q38D + A43D | T180K | G44D + Q105D | K147K | G100K + A43K | S131D |
| 5D03 | Q39K + Q105K | P171D | Q38D + A43D | S162K | G44D + Q105D | A141K | G100K + A43K | F116D |