TABLE 4.
Charged residue pairs at the VH-VL and CH1-CL interfaces were swapped to different combinations to explore the electrostatic steering effect
The amino acid residues in variable regions (VH or VL) are numbered by Kabat numbering system and amino acid residues in constant regions (CH1 or Cκ) are numbered by Eu numbering system.
| Variant | Anti-HER2 humAb4D5-8 HC1 (K392D + K409D) |
Anti-HER2 humAb4D5-8 LC1 |
Anti-HER2 humAb2C4 HC2 (E356K + D399K) |
Anti-HER2 humAb2C4 LC2 |
||||
|---|---|---|---|---|---|---|---|---|
| VH1 | CH1 | VL1 | Cκ | VH2 | CH1 | VL2 | Cκ | |
| V23A | Q39K + Q105K | S183D | Q38D + A43D | S176K | Q39D + Q105D | S183K | Q38K + A43K | S176D |
| V23B | Q39D + Q105K | S183K | Q38K + A43D | S176D | Q39K + Q105D | S183D | Q38D + A43K | S176K |
| V23C | Q39K + Q105D | S183K | Q38D + A43K | S176D | Q39D + Q105K | S183D | Q38K + A43D | S176K |
| V23D | Q39D + Q105D | S183K | Q38K + A43K | S176D | Q39K + Q105K | S183D | Q38D + A43D | S176K |